| dc.description.abstract | Antibiotic resistance is a public health problem. The emergence of carbapenemase-
producing Enterobacterales (CPE) infections is a concern, particularly in Senegal. (1) Methods:
Between January 2019 and July 2022, 240 isolates of enterobacteria resistant to third-generation
cephalosporins and imipenem from biological samples from Fann Hospital (Dakar) and Hôpital
Paix (Ziguinchor) were selected. The isolates were identified by MALDI-TOF mass spectrometry,
and susceptibility tests were performed by the disk diffusion method. Antibiotic-resistance genes
for class A beta-lactamases, carbapenemases, and plasmid resistance to colistin resistance (mcr-1–8)
were screened by RT-PCR. (2) Results: The 240 enterobacteria were composed of: Escherichia coli
(60.83%), Klebsiella pneumoniae (21.67%), Enterobacter cloacae (13.75%), Citrobacter freundii (2.08%), Serra-
tia marcescens (0.83%), Klebsiella aerogenes (0.42%), and Proteus mirabilis (0.42%). Class A beta-lactamase
genes were found in 229 isolates (70.41% blaTEM, 37.5% blaSHV, 83.75% blaCTX-A, and 0.42% blaCTX-B).
The carbapenemase genes blaOXA-48 and blaNDM were found in 25 isolates, including 14 isolates with
blaOXA-48, 13 isolates with blaNDM, and 2 isolates with both genes simultaneously. The mcr-8 gene was
found in one isolate of E. cloacae. (3) Conclusions: The epidemiology of antibiotic-resistance genes in
enterobacteria in Senegal shows the emergence of CPEs. This phenomenon is worrying, and rigorous
surveillance is necessary to avoid further spread. | en_US |
